Journal: GMS Infectious Diseases

Article Title: TGFβ depletion does neither modulate acute E. coli-induced inflammatory immune responses nor impair the protective effect by chronic filarial infection

doi: 10.3205/id000044

Figure Lengend Snippet: (A) Kinetic of the body temperature after intraperitoneal injection of 8.5x10 8 –1.0x10 9 cfu E. coli K12 in chronic L. sigmodontis (L.s.)-infected BALB/c mice (L.s. Isotype: n=20; L.s. anti-TGFβ: n=20) and uninfected controls that received either anti-TGFβ or an isotype control before E. coli challenge (Isotype: n=18; anti-TGFβ: n=17. (B) Peritoneal bacterial load [cfu], (C) absolute cell number of CD11b + F4/80 + macrophages as well as (D) the frequency of macrophages expressing RELMα and macrophage mean fluorescence intensity (MFI) of (E) RELMα and (F) CD86 six hours after E. coli injection. Pooled data of three independent experiments with at least 6 mice per group. Data is shown as median (A), box and whisker blots with min/max (B–F) and was tested for statistical significance by 2-way ANOVA and Bonferroni post hoc test (A), Kruskal-Wallis test and Dunn’s Multiple Comparison post hoc test (B,C,E–F) or 1-way ANOVA with Tukey’s multiple comparisons test (D).

Article Snippet: Ninety-day- L. sigmodontis -infected mice and age-matched controls were intraperitoneally injected with 8.5x10 8 –1.0x10 9 cfu of E. coli K12 (ATCC 25922) in 200 μL sterile LB medium and monitored for 6 h. Body temperature was taken hourly using an infra-red thermometer.

Techniques: Injection, Infection, Control, Expressing, Fluorescence, Whisker Assay, Comparison

Journal: GMS Infectious Diseases

Article Title: TGFβ depletion does neither modulate acute E. coli-induced inflammatory immune responses nor impair the protective effect by chronic filarial infection

doi: 10.3205/id000044

Figure Lengend Snippet: (A) Serum and (B) peritoneal cytokine/chemokine levels six hours after E. coli injection. (C,D) Relative and absolute number of peritoneal CD11b + Ly6G – Ly6C ++ monocytes, (E,F) CD11b + Ly6G + Ly6C + neutrophils and CD11b + F4/80 med SiglecF + eosinophils (G,H) six hours after E. coli injection in mice that were infected for 90 days with L. sigmodontis and uninfected controls and received either anti-TGFβ or an isotype control before E. coli challenge. Data represent three pooled independent experiments with at least six mice per group. Data are shown as mean +SEM (A,B) and median (C–H) and was tested for statistical significance by Kruskal-Wallis test and Dunn’s Multiple Comparison post hoc test.

Article Snippet: Ninety-day- L. sigmodontis -infected mice and age-matched controls were intraperitoneally injected with 8.5x10 8 –1.0x10 9 cfu of E. coli K12 (ATCC 25922) in 200 μL sterile LB medium and monitored for 6 h. Body temperature was taken hourly using an infra-red thermometer.

Techniques: Injection, Infection, Control, Comparison

Journal: eLife

Article Title: Circular synthesized CRISPR/Cas gRNAs for functional interrogations in the coding and noncoding genome

doi: 10.7554/eLife.42549

Figure Lengend Snippet:

Article Snippet: 5x KCM buffer (0.5M KCl, 0.15M CaCl 2 , 0.25M MgCl 2 ), Escherichia coli strain K12 CJ236 (NEB, E4141), SOC outgrowth medium (ThermoFisher Scientific, 15544034), LB-agar plates supplemented with 100 µg/ml ampicillin (Roth, K029.2).

Techniques: Recombinant, Gel Extraction, Plasmid Preparation, Electroporation, Software

Journal:

Article Title: Solid-Phase Capture of Proteins, Spores, and Bacteria †

doi: 10.1128/AEM.67.3.1300-1307.2001

Figure Lengend Snippet: Antibodies investigated for cross-reactivity

Article Snippet: E. coli K12 , Cells , Mouse , 1103/77 , E950831 , Biogenesis Inc. d.

Techniques:

Journal:

Article Title: Solid-Phase Capture of Proteins, Spores, and Bacteria †

doi: 10.1128/AEM.67.3.1300-1307.2001

Figure Lengend Snippet: List of antibodies and their modifications used to capture BSA, OVA, B. globigii spores, and E. coli O157:H7

Article Snippet: E. coli K12 , Cells , Mouse , 1103/77 , E950831 , Biogenesis Inc. d.

Techniques:

Journal:

Article Title: Solid-Phase Capture of Proteins, Spores, and Bacteria †

doi: 10.1128/AEM.67.3.1300-1307.2001

Figure Lengend Snippet: Standard curve of capture of B. globigii spores in PBST (A) and E. coli O157:H7 in meat extract and PBST (B) using 3-mm-diameter PEG-modified glass beads. Standard errors of the means are masked by the symbols. The y axis indicates absorbance.

Article Snippet: E. coli K12 , Cells , Mouse , 1103/77 , E950831 , Biogenesis Inc. d.

Techniques: Modification

Journal:

Article Title: Solid-Phase Capture of Proteins, Spores, and Bacteria †

doi: 10.1128/AEM.67.3.1300-1307.2001

Figure Lengend Snippet: Storage and blocking buffers used to test 3-mm-diameter anti- E. coli O157:H7 PEG-modified glass beads

Article Snippet: E. coli K12 , Cells , Mouse , 1103/77 , E950831 , Biogenesis Inc. d.

Techniques: Blocking Assay

Journal:

Article Title: Solid-Phase Capture of Proteins, Spores, and Bacteria †

doi: 10.1128/AEM.67.3.1300-1307.2001

Figure Lengend Snippet: Effect of blocking buffers on signal generation using 103 total E. coli O157:H7 cells over time stored at 25°C (⧫) and 7°C (◊). Standard errors of the means are masked by the symbols.

Article Snippet: E. coli K12 , Cells , Mouse , 1103/77 , E950831 , Biogenesis Inc. d.

Techniques: Blocking Assay

Journal:

Article Title: Solid-Phase Capture of Proteins, Spores, and Bacteria †

doi: 10.1128/AEM.67.3.1300-1307.2001

Figure Lengend Snippet: Confocal image of captured E. coli O157:H7 cells. Live cells are stained green, and dead cells are stained red. Slides were coated with PEG and anti-E. coli O157:H7 Ab.

Article Snippet: E. coli K12 , Cells , Mouse , 1103/77 , E950831 , Biogenesis Inc. d.

Techniques: Staining

Journal:

Article Title: Exogenous Glutathione Completes the Defense against Oxidative Stress in Haemophilus influenzae

doi: 10.1128/JB.185.5.1572-1581.2003

Figure Lengend Snippet: (a) Partial restriction enzyme map of the nontypeable H. influenzae 3.7-kb genomic DNA fragment cloned by Maciver and Hansen (41), containing ORF HI0571 and ORF HI0572, which expresses transferrin-binding activity in E. coli. The arrows indicate the direction of transcription. (b and c) A 3.9-kb H. influenzae Rd DNA fragment (b) was generated by PCR in order to clone the 1,427-bp MfeI-fragment (c) into EcoRI-linearized pUC18 plasmid DNA (pUC-HI0572).

Article Snippet: E. coli HB101 (Bio-Rad, Hercules, Calif.) was used for propagation of the plasmid vector pUC18 (Gibco-BRL, Rockville, Md.) as well as derivatives of this plasmid.

Techniques: Clone Assay, Binding Assay, Activity Assay, Generated, Plasmid Preparation

Journal:

Article Title: Exogenous Glutathione Completes the Defense against Oxidative Stress in Haemophilus influenzae

doi: 10.1128/JB.185.5.1572-1581.2003

Figure Lengend Snippet: Effect of the introduction of the pUC-HI0572 plasmid on the susceptibilities of wild-type (K-12), acatalasemic (UM2), and Ahp-negative (TA4315) E. coli strains to H2O2 (A) and t-BuOOH (B).

Article Snippet: E. coli HB101 (Bio-Rad, Hercules, Calif.) was used for propagation of the plasmid vector pUC18 (Gibco-BRL, Rockville, Md.) as well as derivatives of this plasmid.

Techniques: Plasmid Preparation

Journal:

Article Title: Exogenous Glutathione Completes the Defense against Oxidative Stress in Haemophilus influenzae

doi: 10.1128/JB.185.5.1572-1581.2003

Figure Lengend Snippet: GSH-dependent peroxidase and catalase activities monitored in log-phase lysates derived from wild-type and mutant E. coli strains transformed with the pUC18 (control) or pUC-HI0572 plasmid a

Article Snippet: E. coli HB101 (Bio-Rad, Hercules, Calif.) was used for propagation of the plasmid vector pUC18 (Gibco-BRL, Rockville, Md.) as well as derivatives of this plasmid.

Techniques: Derivative Assay, Mutagenesis, Transformation Assay, Plasmid Preparation

Journal: Scientific Reports

Article Title: Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor

doi: 10.1038/srep11222

Figure Lengend Snippet: ( a ) CFU counts and relative proportion (%) of E. coli and S. oneidensis strains in anode media. ( b ) CFU counts and relative proportion (%) of E. coli and S. oneidensis strains on the anode electrode.

Article Snippet: E. coli K-12 (ATCC #10798) was purchased from the American Type Culture Collection (Manassas, VA).

Techniques:

Journal: Scientific Reports

Article Title: Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor

doi: 10.1038/srep11222

Figure Lengend Snippet: Selected gene expression changes in E. coli induced by S. oneidensis for energy metabolism.

Article Snippet: E. coli K-12 (ATCC #10798) was purchased from the American Type Culture Collection (Manassas, VA).

Techniques: Gene Expression

Journal: Scientific Reports

Article Title: Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor

doi: 10.1038/srep11222

Figure Lengend Snippet: Glucose is fermented by E. coli to yield metabolites such as formate, which is taken up by S. oneidensis as electron donor. Flavin mediator molecules are secreted to facilitate electron movement to the external charge collecting electrode to derive energy in MFCs. Flavins can also be taken up by E. coli to facilitate its respiration on electrodes as E. coli does not secrete flavins.

Article Snippet: E. coli K-12 (ATCC #10798) was purchased from the American Type Culture Collection (Manassas, VA).

Techniques:

Journal: Scientific Reports

Article Title: Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor

doi: 10.1038/srep11222

Figure Lengend Snippet: Bacterial strains used in this study.

Article Snippet: E. coli K-12 (ATCC #10798) was purchased from the American Type Culture Collection (Manassas, VA).

Techniques:

Journal: Scientific Reports

Article Title: Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor

doi: 10.1038/srep11222

Figure Lengend Snippet: ( a ) Photograph of MFC setup. ( b ) Graphic of mutualistic interactions between E. coli and S. oneidensis in MFC setup.

Article Snippet: E. coli K-12 (ATCC #10798) was purchased from the American Type Culture Collection (Manassas, VA).

Techniques:

Journal: International Journal of Biochemistry and Molecular Biology

Article Title: Comparison of the functions of glutathionylspermidine synthetase/amidase from E. coli and its predicted homologues YgiC and YjfC

doi:

Figure Lengend Snippet: Analysis of thiol content in E. coli cells. Analysis of the thiol content of wild type and gsp, yjfC, and ygiC gene knockout E. coli strains grown in LB media to stationary phase under anaerobic conditions was performed using HPLC. Labeled peaks represent DTNB derivatives of G-Sp and GSH. Peak immediately followed GSH (14 min) corresponds to DTNB derivative of γ-glutamylcysteine. Peaks at 18 min and 21 min are 2-nitro-5-thiobenzoate and the excess of DTNB correspondently.

Article Snippet: Materials E. coli K-12 genomic DNA was from ATCC (Manassas).

Techniques: Gene Knockout, Labeling